Exploiting Issatchenkia orientalis SD108 for succinic acid production

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Exploiting Issatchenkia orientalis SD108 for succinic acid production

BACKGROUND Recent advances in synthesizing valuable chemicals such as organic acids from low-cost renewable biomass through microbial fermentation have attracted great attention. However, the toxicity of organic acids presents a key challenge to the development of an economically viable fermentation process. Therefore, a platform organism that not only produces organic acids but also tolerates ...

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Purification and properties of glutathione transferase from Issatchenkia orientalis.

Glutathione transferase (GST) (EC 2.5.1.18) was purified from a cell extract of Issatchenkia orientalis, and two GST isoenzymes were isolated. They had molecular weights of 37,500 and 40,000 and were designated GST Y-1 and GST Y-2, respectively. GST Y-1 and GST Y-2 gave single bands with molecular weights of 22,000 and 23,500, respectively, on sodium dodecyl sulfate-polyacrylamide gel electroph...

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Production of succinic acid by bacterial fermentation

Succinic acid produced by various microorganisms can be used as a precursor of many industrially important chemicals in food, chemical and pharmaceutical industries. The assessment of raw material cost and the estimation of the potential market size clearly indicate that the current petroleum-based succinic acid process will be replaced by the fermentative succinic acid production system in the...

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METABOLIC CAPABILITIES OF Actinobacillus succinogenes FOR SUCCINIC ACID PRODUCTION

Attention has been focused on microbial succinic acid production as an alternative for conventional chemical synthesis that is associated with environmental pollution. A metabolic model for Actinobacillus succinogenes 130Z was developed with a mixture of glucose and xylose as substrate. The metabolic fluxes during succinicate production were determined using flux balance analysis by linear prog...

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Degradation of malic acid by Issatchenkia orientalis KMBL 5774, an acidophilic yeast strain isolated from Korean grape wine pomace.

Several yeast strains degrading malic acid as a sole carbon and energy source were isolated from Korean wine pomace after enrichment culture in the presence of malic acid. Among them, the strain designated as KMBL 5774 showed the highest malic acid degrading ability. It was identified as Issatchenkia orientalis based on its morphological and physiological characteristics as well as the nucleoti...

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ژورنال

عنوان ژورنال: Microbial Cell Factories

سال: 2014

ISSN: 1475-2859

DOI: 10.1186/s12934-014-0121-4